WebThe pools were eluted with 3.2 ml of TBS pH 7.5. These two purified pools were used for the off-rate selection in the second round of phage display at concentrations of approximately 390 µM for selection variant 1 (competing for binding to RBP-Fc) and 450 µM for selection variant 2 (competing for binding to RBP-YFP). WebJul 21, 2015 · You add exemptions to the pool of addresses from one DHCP server on the other DHCP server and vice versa. For example. DHCP server one scope serves 192.168.0.10 - 20 (add exemptions for 192.168.0.21 - 40) DHCP server two scope serves 192.168.0.21 - 40 (add exemptions for 192.168.0.1 - 20)
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